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Octavio García-Depraect, Víctor F. Diaz-Cruces , Eldon R. Rene , Roberto Castro-Munoz , Elizabeth Leon-Becerril.
Bioresource Technology Reports
The aim of this work was to evaluate the feasibility of preserving and readily supplying a hydrogenogenic consortium by refrigeration at 4 °C for a prolonged time of up to 30 months. Hydrogen production capacity during storage was evaluated in batch dark fermentation tests using lactose and compared to that of fresh inoculum. Throughout the storage, the refrigerated consortium maintained its functionality after reactivation. Following 730 days of storage, the activated consortium retained 92.1% of the initial hydrogen production capacity, yielding 2.1 mol H2/mol lactose. Molecular analyses suggested that microbial composition changed over time, being the process driven by microorganisms present in the mother culture, particularlyClostridiumand other non‑hydrogen-producing bacteria such as lactic acid bacteria, which were able to survive and recover from storage. The proposed preservation approach could provide active hydrogenogenic biomass for being used in practice to restart-up, bioaugment and operate reactors under seasonal conditions.
Biorreactor 3L Applikon